A simplified serum - neutralization test based on enhanced green fluorescent 1 protein - tagged Classical swine fever virus
نویسندگان
چکیده
A simplified serum-neutralization test based on enhanced green fluorescent 1 protein-tagged Classical swine fever virus 2 3 Yongfeng Li, Liang Shen, Yuan Sun, Jin Yuan, Junhua Huang, Chao Li, Su Li, Yuzi 4 Luo, Hua-Ji Qiu* 5 6 State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 7 Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China 8 9 Running title: Simplified serum-neutralization test for CSFV 10 11 Number of words: Abstract: 74 words; Text: 1083 words 12 Number of Tables and Figures: 1 table and 2 figures 13 14 * Corresponding author. 15 Dr. Hua-Ji Qiu 16 State Key Laboratory of Veterinary Biotechnology 17 Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences 18 427 Maduan Street Harbin 150001, Heilongjiang, China 19 Phone: +86 189 4606 6041 20 Fax: +86-451-5199 7170 21 E-mail: [email protected] 22 Copyright © 2013, American Society for Microbiology. All Rights Reserved. J. Clin. Microbiol. doi:10.1128/JCM.00859-13 JCM Accepts, published online ahead of print on 22 May 2013
منابع مشابه
Simplified serum neutralization test based on enhanced green fluorescent protein-tagged classical swine fever virus.
The neutralization immunofluorescence test (NIFT), currently used for detecting neutralizing antibodies (NAbs) against classical swine fever virus (CSFV), is time-consuming. Here, a simplified neutralization test based on enhanced green fluorescent protein (EGFP)-tagged CSFV (EGFP-NT) was developed for direct detection of anti-CSFV NAbs without immunostaining. The relative sensitivity and speci...
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